Anti-Actin, Cardiac antibody, Mouse monoclonal
SIGMA/A9357 - clone AC1-20.4.2, purified from hybridoma cell culture
Synonym: Anti-ACTC; Anti-ACTC1; Anti-Actin alpha cardiac muscle 1; Anti-CMD1R; Anti-CMH11
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rat heart sections with 10 μg/mL Monoclonal Anti-Actin, Cardiac, Clone: AC1-20.4.2 (Cat. No. A9357) using biotin/ExtrAvidin®-Peroxidase.
Immunoblotting Whole extract of rat heart was separated on SDS-PAGE and probed with Monoclonal Anti-Actin, Cardiac Clone: AC1-20.4.2 (Cat. No. A9357). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. 10 μg/mL antibody 2. 5 μg/mL antibody 3. 2.5 μg/mL antibody
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | AC1-20.4.2, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~42 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, bovine, chicken, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using digested rat heart tissue | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | P68032  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) Western Blotting (1 paper) |
| Application: | Monoclonal Anti-Actin, Cardiac antibody is suitable for use in immunocytochemistry, immunoblot (approx. 42 kDa), indirect ELISA, and western blot. The antibody may also be used in immunohistochemistry (using formalin-fixed, paraffin-embedded sections, or using a conc. of 10-20 μg/mL in digested rat heart tissue). |
| Application: | Protein samples isolated from cardiac sections or cardiac myocytes were subjected to western blot using monoclonal mouse anti-actin antibody as the primary at a dilution of 1:2000. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Actins are cytoskeletal proteins that are widely expressed in eukaryotes. These proteins primarily regulate various cellular functions such as muscle contraction, mRNA synthesis and apoptosis among many others. ACTC1 is the predominant actin isoform form in adult cardiac tissues and alterations in this isoform have been associated with hypertrophic cardiomyopathy and congenital heart disease . Monoclonal Anti-Actin, Cardiac antibody is specific for human, bovine, chicken, rat, and mouse cardiac α actin. |
| Immunogen: | synthetic NH2-terminal decapeptide of cardiac isoform of actin. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |