L-Amino Acid Oxidase from Crotalus adamanteus
SIGMA/A9378 - Type IV, ≥4.0 units/mg protein, aqueous suspension
Synonym: L-AAO; L-Amino acid:oxygen oxidoreductase (deaminating)
CAS Number: 9000-89-9
EC Number: 232-564-0
MDL Number: MFCD00131454
Product Type: Chemical
| concentration | ≥5.0 mg/mL |
| contains | toluene as preservative |
| form | aqueous suspension |
| mol wt | ~130 kDa |
| Quality Level | 200  |
| solubility | H2O: soluble 1.0 mg/mL, clear |
| specific activity | ≥4.0 units/mg protein |
| storage temp. | 2-8°C |
| type | Type IV |
| Application: | L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays. 35S dimethylsulfoniopropionat |
| Biochem/physiol Actions: | L-Amino acid oxidase is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70,000 Da. Each molecule of holoenzyme has two FAD molecules. It is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. Optimum pH is approximately 7.5.The enzyme may be reversibly inactivated by incubation in phosphate buffer, pH 7.5 at 38 °C. L-amino acid oxidase is involved in various metabolic pathways such as alanine and aspartate metabolism, methionine metabolism, valine, leucine and isoleucine degradation, tyrosine metabolism, phenylalanine metabolism, tryptophan metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and alkaloid biosynthesis. It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney and liver. |
| Packaging: | 2, 5, 10 mg in glass bottle |
| Packaging: | Package size based on protein content |
| Preparation Note: | Dissolves in water at 1 mg/mL concentration to form a clear solution. |
| Unit Definition: | One Unit oxidizes one micromole of |
| Symbol |  GHS06 |
| Signal word | Danger |
| Hazard statements | H300 + H310 + H330 |
| Precautionary statements | P262 - P280 - P301 + P310 + P330 - P302 + P352 + P310 - P304 + P340 + P310 |
| Hazard Codes | T+ |
| Risk Statements | 26/27/28 |
| Safety Statements | 36/37/39-45 |
| RIDADR | UN 3462 6.1 / PGI |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥4.0 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.4.3.2 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |