Actinomycin D

SIGMA/A9415 - from Streptomyces sp., suitable for cell culture, ≥95%

Synonym: Cosmegen; Meractinomycin; Actinomycin C₁; Actinomycin IV; Dactinomycin

CAS Number: 50-76-0
Empirical Formula (Hill Notation): C₆₂H₈₆N₁₂O₁₆
Molecular Weight: 1255.42
EC Number: 200-063-6
MDL Number: MFCD00005033
Linear Formula: C₆₂H₈₆N₁₂O₁₆
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-A9415-2MG	2 mg	$85.10 1/EA	Add To Favorites
45-A9415-5MG	5 mg	$131.00 1/EA	Add To Favorites
45-A9415-10MG	10 mg	$251.00 1/EA	Add To Favorites
45-A9415-25MG	25 mg	$549.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A9415-10MG

Properties
Descriptions
Safety Info.
Basic Data

antibiotic activity spectrum	neoplastics
assay	≥95%
biological source	Streptomyces sp.
color	red, powder
form	powder
InChI	1S/C62H86N12O16/c1-27(2)42-59(84)73-23-17-19-36(73)57(82)69(13)25-38(75)71(15)48(29(5)6)61(86)88-33(11)44(55(80)65-42)67-53(78)35-22-21-31(9)51-46(35)64-47-40(41(63)50(77)32(10)52(47)90-51)54(79)68-45-34(12)89-62(87)49(30(7)8)72(16)39(76)26-70(14)58(83)37-20-18-24-74(37)60(85)43(28(3)4)66-56(45)81/h21-22,27-30,33-34,36-37,42-45,48-49H,17-20,23-26,63H2,1-16H3,(H,65,80)(H,66,81)(H,67,78)(H,68,79)/t33-,34-,36+,37+,42-,43-,44+,45+,48+,49+/m1/s1
InChI key	RJURFGZVJUQBHK-IIXSONLDSA-N
mode of action	DNA synthesis \| interferes
mp	251-253
Quality Level	300
SMILES string	CC(C)[C@H]1NC(=O)[C@@H](NC(=O)c2ccc(C)c3OC4=C(C)C(=O)C(N)=C(C(=O)N[C@H]5[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]6CCCN6C(=O)[C@H](NC5=O)C(C)C)C4=Nc23)[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]7CCCN7C1=O
solubility	ethanol, DMSO: soluble (Stable in aqueous solutions at 2-8 °C.)
storage temp.	2-8°C
technique(s)	cell culture \| mammalian: suitable

Application:	Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation with wide-ranging applications ranging from a selection agent in cell culture, use in studies of suppressing HIV-replication and programmed cell death of PC12 cells.. Recommended for us in cell culture applications at 1μg/mL.
Biochem/physiol Actions:	Actinomycin D inhibits the proliferation of cells by forming a stable complex with double-stranded DNA, inhibiting DNA-primed RNA synthesis and causing single-stranded breaks in DNA. It has been shown to be an inhibitor of the minus-strand transfer step in reverse transcriptase.
Caution:	This product is hygroscopic and light-sensitive. If stored protected from light and moisture at 2-8°C, it remains unchanged, by HPLC testing, for at least 15 months. Unused dilute solutions of Actinomycin D are extremely sensitive to light and tend to adsorb to plastic and glass and should be discarded. However, frozen aliquots of concentrated stock solution are stable for at least a month.
General description:	Chemical structure: peptide
Other Notes:	Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.
Packaging:	2, 5, 10, 25 mg in glass bottle
Preparation Note:	Actinomycin D is sold as red, shiny crystals and is soluble in acetonitrilie or acetone at 10 mg/mL and in DMSO at a minimum of 1 mg/mL. Some references also show slight solubility in water of 0.5 mg/mL.

Symbol	GHS06,GHS08
Signal word	Danger
Hazard statements	H300 - H350 - H360FD
Precautionary statements	P201 - P202 - P264 - P270 - P280 - P301 + P310
Hazard Codes	T+
Risk Statements	28
Safety Statements	22-28-36/37-45
RIDADR	UN 3462 6.1 / PGII
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥95%
mp	251-253
Storage Temp.	2-8°C
UNSPSC	51282001

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