Bovine Serum Albumin
SIGMA/A9430 - heat shock fraction, low endotoxin, pH 7, ≥98%
Synonym: Albumin bovine serum; BSA; Bovine albumin
CAS Number: 9048-46-8
EC Number: 232-936-2
MDL Number: MFCD00130384
Product Type: Chemical
assay | ≥98% |
biological source | bovine |
foreign activity | BT virus, none detected |
VSV virus, none detected | |
form | lyophilized powder |
impurities | ≤1.0 EU/mg endotoxin |
loss | ≤5% |
mol wt | ~66 kDa |
origin | USA origin |
packaging | poly bottle of |
pH | 7 |
purified by | heat shock fractionation |
Quality Level | 200 |
solubility | water: soluble (40 mg/ml) |
storage temp. | 2-8°C |
technique(s) | ELISA: suitable |
microbiological culture: suitable | |
UniProt accession no. | P02769 |
Application: | This A9430 BSA product has been used in various systems and studies, including: • HK-2 cells: Ishola, D.A., Jr. et al., Kidney International, 70, 724-731 (2006). • Mice: For vasoconstriction studies: Szczepaniak, W.S. et al., Am. J. Physiol. Lung Cell. Mol. Physiol., 299(1), L137-L145 (2010) For studies of protein overload nephropathy: a) Chang, A.M. et al., Nephrol. Dial. Transplant., 27, 1330-1343 (2012) b) Wong, D.W.L. et al., Cell Death Dis., 7, e2155 (2016). For protein overload related to resistance to loop diuretics: Zhuang, Y., Jia, Z., et al., Oncotarget, 8(44), 75808-75821 (2017). |
Biochem/physiol Actions: | Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies. |
Packaging: | 25, 100 g in poly bottle |
Preparation Note: | Prepared using heat shock fractionation |
Preparation Note: | Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration. |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥98% |
Storage Temp. | 2-8°C |
UNSPSC | 12352202 |