Ammonium chloride
SIGMA/A9434 - for molecular biology, suitable for cell culture, ≥99.5%
Synonym: Salmiac
CAS Number: 12125-02-9
Empirical Formula (Hill Notation): H4ClN
Molecular Weight: 53.49
EC Number: 235-186-4
MDL Number: MFCD00011420
Linear Formula: NH4Cl
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: A9434-500G
This image depicts SKU: A9434-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: A9434-1KG
This image depicts SKU: A9434-1KG
| anion traces | phosphate (PO43-): ≤2 ppm |
| sulfate (SO42-): ≤20 ppm | |
| assay | ≥99.5% |
| cation traces | Fe: ≤2 ppm |
| heavy metals (as Pb): ≤5 ppm | |
| foreign activity | DNase, RNase, protease, none detected |
| form | powder or crystals |
| grade | for molecular biology |
| InChI | 1S/ClH.H3N/h1H;1H3 |
| InChI key | NLXLAEXVIDQMFP-UHFFFAOYSA |
| mp | 340 °C (subl.) (lit.) |
| Quality Level | 200  |
| SMILES string | N.Cl |
| technique(s) | cell culture | mammalian: suitable |
| vapor density | 1.9 (vs air) |
| vapor pressure | 1 mmHg ( 160.4 °C) |
| Application: | Ammonium chloride has been used: • as an inhibitor of endosome acidification in vero cells • as an autophagy inhibitor in vascular smooth muscle cell (VSMC) • in the depletion and lysis of erythrocytes in testis parenchyma homogenate |
| Biochem/physiol Actions: | Ammonium chloride is one of the three principal components of the nitrogen cycle. Ammonium chloride administration promotes creatinine and urea clearance andinduces metabolic acidosis in mice. Excess of ammonium chloride inhibits Kreb′s pathway in brain and depletes ATP in astrocytes. Ammonium chloride inhibits acidification in the endosome-lysosome system. Ammonium chloride is a hemolysing agent used for the lysis of erythrocytes. |
| Packaging: | 1 kg in poly bottle |
| Packaging: | 500 g in poly bottle |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 - H319 |
| Precautionary statements | P264 - P270 - P280 - P301 + P312 - P305 + P351 + P338 - P337 + P313 |
| Hazard Codes | Xn |
| Risk Statements | 22-36 |
| Safety Statements | 22 |
| RIDADR | UN 3077 9 / PGIII |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.5% |
| mp | 340 °C (subl.) (lit.) |
| UNSPSC | 12352302 |