Anti-Goat/Sheep IgG−Peroxidase antibody, Mouse monoclonal
SIGMA/A9452 - clone GT-34, purified from hybridoma cell culture
Synonym: Monoclonal Anti-Goat/Sheep IgG
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human Tonsil tissue sections were stained with 1:25 Anti-Human IgG (whole molecule) antibody produced in Goat (Cat. No. I1011), followed by 1:100 Monoclonal Anti-Goat/Sheep IgG-Peroxidase antibody produced in Mouse, Clone: GT-34 (Cat. No. A9452).
| antibody form | purified from hybridoma cell culture |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | GT-34, monoclonal |
| conjugate | peroxidase conjugate |
| form | lyophilized powder |
| isotype | IgG1 |
| packaging | vial of 0.5 mL |
| Quality Level | 200  |
| should not react with | rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human |
| species reactivity | sheep, goat, bovine |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:30,000 |
| dot blot: 1:160,000 (chemiluminescent) | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 |
| Application: | ELISA using HRP-conjugated monoclonal anti-goat/sheep IgG as the secondary was performed on human T cells. |
| Application: | Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody has been used for western blot (1:5,000) and ELISA. The product can also be used for dot blot (1:30,000) and IHC (1:100). |
| Biochem/physiol Actions: | IgG activates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a key role in antibody-dependent cell-mediated cytotoxicity (ADCC). IgG enables transplacental transport. It has the longest serum half life. It might be associated with allergy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG is the most abundant antibody in human serum.It is found in blood and secreted by the B cells. It consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are connected by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4. |
| General description: | IgGs are glycoprotein antibodies that modulate several immune responses. Goat or sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-goat/sheep IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Mouse monoclonal anti-goat/sheep IgG-peroxidase antibody shows strong cross-reactivity with sheep IgG and cross-reacts with bovine IgG. No cross-reaction of the unconjugated monoclonal antibody is observed with human serum and tissue components. Moreover, the antibody does not react with the following species: guinea pig, rat, mouse, rabbit, horse, dog, chicken, pig and cat. |
| General description: | The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2. |
| Physical form: | Lyophilized from 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |