Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse
SIGMA/A9469 - clone M2, purified immunoglobulin, buffered aqueous glycerol solution
Synonym: Monoclonal ANTI-FLAG® M2; Anti-ddddk; Anti-dykddddk
Product Type: Chemical
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| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | M2, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | alkaline phosphatase conjugate |
| form | buffered aqueous glycerol solution |
| immunogen sequence | DYKDDDDK |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | all |
| storage temp. | −20°C |
| technique(s) | indirect ELISA: 1:20,000 |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse has been used: • in direct tissue blot immunoassay of sweet orange petioles samples • in screening internalization of delta opioid receptor • for screening cell-free protein expression using ELISA |
| General description: | Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase is a purified IgG 1 mouse antibody covalently conjugated to calf intestinal alkaline phosphatase (AP). The antibody conjugate binds to FLAG® fusion proteins and will recognize the FLAG® epitope at any position in the fusion protein (N-terminal, Met-N-terminal, C-terminal or internal FLAG® peptides). |
| Legal Information: | ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Solution in Tris buffered saline containing 50% glycerol plus stabilizer and preservative |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106514 |