Anti-Glucose-6-Phosphate Dehydrogenase (G-6-PDH) antibody produced in rabbit
SIGMA/A9521 - IgG fraction of antiserum, lyophilized powder
MDL Number: MFCD00162329
Product Type: Chemical
Western Blotting JOURNAL CITATION: Dynamic rerouting of the carbohydrate flux is key to counteracting oxidative stress. By: Ralser, M., Wamelink, M. M., et al. in J Biol, 2007. PubMed ID: 18154684 Image collected and cropped by CiteAb from the following publication, (http://jbiol.biomedcentral.com/articles/10.1186/jbiol61), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
ELISA Anti-Glucose-6-Phosphate Dehydrogenase (G-6-PDH) antibody produced in Rabbit (Cat. No. A9521), followed by 1:1,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545), was serially diluted to detect Glucose-6-Phosphate Dehydrogenase by indirect ELISA.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| Quality Level | 200  |
| shipped in | ambient |
| species reactivity | yeast |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoelectrophoresis: suitable |
| indirect ELISA: 1:15,000-1:30,000 |
| Application: | Anti-Glucose-6-Phosphate Dehydrogenase (G-6-PDH) antibody has been used to detect the immunoreactive mass of glyceraldehyde 3-phosphate dehydrogenase (GAPDH). It has also been used in western blotting. |
| Application: | Rabbit Anti-Glucose-6-Phosphate Dehydrogenase (G-6-PDH) antibody has been used for western blot assays. The antibody can also be used for indirect ELISA (1:15,000-1:30,000) and immunoelectrophoresis. |
| Application: | Yeast cell extracts were analyzed by western blot and equal loading was determined by probing with rabbit anti-Glucose-6-Phosphate Dehydrogenase antibody. |
| Biochem/physiol Actions: | NADPH (nicotinamide adenine dinucleotide phosphate), produced by glucose-6-phosphate dehydrogenase (G6PD) is an electron donor molecule, that helps in neutralizing harmful oxidizing agents. Deficiency in G6PD shows symptoms, that includes neonatal jaundice and acute episodic hemolysis. This enzyme guards the red blood cells against oxidative challenges. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | G-6-PDH catalyzes the NADP+(or NAD+)-dependent oxidation of D-glucose-6-phosphate to 6-phospho-D-glucono-1,5-l Yeast G-6-PDH is often used as a loading control in several immunoassays. |
| General description: | Glucose-6-phosphate dehydrogenase (G6PD) is a cytosolic enzyme. It synthesizes NADPH (nicotinamide adenine dinucleotide phosphate). The G6PD gene is located on human chromosome X. It spans 18.5 kb and has 13 exons. |
| General description: | The product is specific for G-6-PDH as determined by immunoelectrophoresis. No reaction is observed with other proteins in a crude Baker′s yeast extract. |
| Immunogen: | G-6-PDH from Baker′s yeast S. cerevisiae. |
| Physical form: | Lyophilized from 0.01 M phosphate buffered saline. |
| Reconstitution: | Reconstitute with 2mL deionized water. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |