Amphotericin B solubilized
SIGMA/A9528 - powder, γ-irradiated, BioXtra, suitable for cell culture
Synonym: Amphotericin B from Streptomyces sp.; Fungizone
CAS Number: 1397-89-3
Empirical Formula (Hill Notation): C47H73NO17
Molecular Weight: 924.08
MDL Number: MFCD00877763
Linear Formula: C47H73NO17
Product Type: Chemical
antibiotic activity spectrum | fungi |
yeast | |
form | powder |
InChI | 1S/C47H73NO17/c1-27-17-15 |
InChI key | APKFDSVGJQXUKY-INPOYWNPSA |
mode of action | cell membrane | interferes |
product line | BioXtra |
Quality Level | 300 |
SMILES string | C[C@H]1O[C@@H](O[C@@H]2C[ |
sterility | γ-irradiated |
storage temp. | 2-8°C |
technique(s) | cell culture | mammalian: suitable |
Application: | Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium. |
Biochem/physiol Actions: | Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast. |
Caution: | Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years. |
Components: | This product is a colloidal suspension of Amphotericin B, using deoxycholate as the solubilizing agent. The formulation is ~45% Amphotericin B and 35% sodium deoxycholate in sodium phosphate and sodium chloride. |
General description: | Chemical structure: polyene |
Other Notes: | Keep container tightly closed in a dry and well-ventilated place. Product is sensitive to light and moisture. Keep in a dry place. |
Other Notes: | The actual solid weight of the packaged product is calculated according to the UV purity. |
Packaging: | 1, 5 g in glass bottle |
Packaging: | 50, 100, 500 mg in glass bottle |
Packaging: | Solubilized Amphotericin B is packaged in amber glass bottle, with the contents inside an inserted fused cone. |
Preparation Note: | Amphotericin B is soluble at 19.6-20.4 mg/ml in water, at natural pH without heating, giving a yellowish clear to slightly hazy solution. Aqueous solutions cannot be sterile filtered due to poor solubility. If reconstituted in sterile water, there is no need to filter sterilize. It is also soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL). |
Symbol | GHS07 |
Signal word | Warning |
Hazard statements | H335 |
Hazard Codes | Xn |
Risk Statements | 22-36/37/38 |
Safety Statements | 26 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | 2-8°C |
UNSPSC | 12352207 |
Components | Amphotericin B ~ 45 %; Sodium deoxycholate ~ 35 %; Sodium phosphate balance |