Agarose
SIGMA/A9539 - BioReagent, for molecular biology, low EEO
Synonym: 3,6-Anhydro-α-L-galacto-β-D-galactan; Agarose LE
CAS Number: 9012-36-6
EC Number: 232-731-8
MDL Number: MFCD00081294
Product Type: Chemical
A representative image of the product packaging
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: A9539-100G
This image depicts SKU: A9539-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: A9539-50G
This image depicts SKU: A9539-50G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: A9539-250G
This image depicts SKU: A9539-250G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: A9539-500G
This image depicts SKU: A9539-500G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: A9539-10G
This image depicts SKU: A9539-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.
This image depicts SKU: A9539-25G
This image depicts SKU: A9539-25G
| anion traces | sulfate (SO42-): ≤0.15% |
| biological source | algae (marine) |
| EEO | 0.09-0.13 |
| foreign activity | DNase, RNase, none detected |
| form | powder |
| gel strength | ≥1200 g/cm2 (1% gel) |
| grade | for molecular biology |
| impurities | ≤10% moisture content |
| InChI | 1S/C24H38O19/c25-1-5-9(27 |
| InChI key | MJQHZNBUODTQTK-WKGBVCLCSA |
| product line | BioReagent |
| Quality Level | 200  |
| suitability | suitable for electrophoresis |
| suitable for molecular biology | |
| technique(s) | electrophoresis: suitable |
| transition temp | gel point 36 °C ±1.5 °C (1.5% gel) |
| Analysis Note: | The following is a list of properties associated with our agaroses: Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present. Gel strength - the force that must be applied to a gel to cause it to fracture. Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature. Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection. |
| Application: | Agarose has been used: • in gel electrophoresis to analyze the integrity of DNA • to prepare hydrocolloid gels and study the structural influence of gels on the release of carbohydrates • in gel electrophoresis to analyze the integrity of RNA • it is suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID) |
| Biochem/physiol Actions: | Agarose serves as a gelling agent and is extensively used in molecular biology research to separate and analyze nucleic acids by gel electrophoresis or blotting (Northern or Southern). During gelation, agarose polymers combine non-covalently resulting in a network of bundles whose pore sizes decide a gel′s molecular sieving properties. The type and the concentration of agarose influence the rate of migration of a DNA molecule through a gel. However, agarose also has attractive features that bring about a strong interest in its usage in biological applications. It is known to mimic the extracellular matrix which makes it an excellent biomaterial for tissue engineering applications. Due to its great water uptake capability agarose is suitable for cell-encapsulation. Agarose allows regulated permeation for oxygen and nutrients and is useful in cell growth, differentiation, and proliferation. Agarose is used in controlled/localized drug delivery and regenerative medicine, such as neurogenesis, angiogenesis, spermatogenesis, cartilage formation, bone regeneration, wound healing, and artificial pancreas. |
| Features and Benefits: | • Biocompatible • Agarose gels have larger pore sizes than polyacrylamide gels at low concentrations • Unlike polyacrylamide, the consistency of the gels is more solid (but also less elastic) • Possesses low ethidium bromide and SYBR Green background staining |
| General description: | Agarose is a natural polysaccharide isolated from the seaweed genera Gelidium and Gracilaria. Structurally, it is a linear polymer consisting of alternating D-galactose and 3,6-anhydro-L-galactose units. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Packaging: | 10, 25, 50, 100, 250, 500 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41105317 |