Anti-Rat IgG (whole molecule)−Peroxidase antibody produced in rabbit
SIGMA/A9542 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Rabbit Anti-Rat IgG (whole molecule)−HRP
MDL Number: MFCD00162813
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human tonsil tissue sections were stained with 1:100 Rat Anti-Human IgG antibody followed by 1:500 Anti-Rat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A9542).
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with 1:12,000 Monoclonal Anti-Importin α-1 antibody produced in Rat (Cat. No. I9658). The antibody was developed using Anti-Rat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A9542). Lanes 1. 1:5,000 antibody 2. 1:10,000 antibody 3. Negative Control
ELISA Anti-Rat IgG (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A9542) was serially diluted to detect Rat IgG by direct ELISA.
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:40,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 | |
| western blot (chemiluminescent): 1:5,000 using detecting rat primary antibody |
| Application: | Anti-Rat IgG (whole molecule) Peroxidase antibody produced in rabbit has been used in western blotting and enzyme linked immunosorbent assay (ELISA). |
| Biochem/physiol Actions: | IgGs are glycoprotein antibodies that modulate several immune responses. Rat IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rat IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG (Immunoglobulin G) antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. |
| General description: | Immunoglobulins have two heavy chains and two light chains connected by disulfide bond. It is a glycoprotein which mainly helps in immune defense. IgG is a major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c. |
| Immunogen: | purified rat IgG |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Specificity: | Anti-Rat IgG (whole molecule) antibody recognizes rat (immunoglobulin G) IgG and is determined by immunoelectrophoresis (IEP) versus normal rat serum and rat IgG prior to conjugation. |
| Storage and Stability: | For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |