Monoclonal ANTI-FLAG® M2-Cy3™ antibody produced in mouse
SIGMA/A9594 - clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
Synonym: Monoclonal ANTI-FLAG® M2 antibody produced in mouse; Anti-ddddk; Anti-dykddddk
Product Type: Chemical
COS 7 cells transfected with pFLAG-CMV2-BAP plasmid stained with with ANTI-FLAG Cy3 Conjugate (A 9594).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | M2, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | CY3 conjugate |
| form | buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate) |
| immunogen sequence | DYKDDDDK |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | all |
| storage temp. | −20°C |
| technique(s) | direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity. Learn more product details in our FLAG® application portal. |
| General description: | Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins. |
| Legal Information: | ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Cy3 is a trademark of Cytiva |
| Legal Information: | FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Suggested concentration of 1-10 mg/ml for immunocytochemistry. |
| Packaging: | 0.2, 1 mg in poly bottle |
| Physical form: | Solution in phosphate buffered saline plus 1% BSA and preservative |
| Preparation Note: | Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106514 |