α-Actinin from chicken gizzard
SIGMA/A9776 - ~80% α-actinin basis (SDS-PAGE), ammonium sulfate suspension
CAS Number: 11003-00-2
MDL Number: MFCD00130473
Product Type: Chemical
| assay | ~80% α-actinin basis (SDS-PAGE) |
| biological source | chicken gizzard |
| form | ammonium sulfate suspension |
| mol wt | 100 kDa |
| storage temp. | 2-8°C |
| UniProt accession no. | P05094   |
| P12814 |
|
| P20111 |
|
| Q7TPR4 |
|
| Q90734 |
|
| Q9Z1P2 |
| Application: | α-Actinin from chicken gizzard has been used: • as an antigen to coat plates for the capture of anti-actinin antibody using enzyme-linked immunosorbent assay (ELISA) from tumor cell line clones • in in vitro motility experiments to test its effect on actin filament movement • to coat cantilevers for strengthening fluorescently labeled actin filaments in force measurement studies |
| Biochem/physiol Actions: | α-Actinin binds actin and has the ability to crosslink actin cytoskeleton. It also plays a key role in the structural maintenance of the Z-disk of striated muscle. |
| General description: | α-Actinin exists as a rod-shaped antiparallel dimer with two elongated subunits. The domain region contains an N-terminal actin-binding domain with tandem calponin homology domains, a central tandem 3-helix motifs, and EF-hand motifs at C-terminus. It belongs to the spectrin superfamily and is localized in actin structures. |
| Packaging: | 5 mg in poly bottle |
| Packaging: | Package size based on protein content |
| Physical form: | Suspension in 2 M (NH4)2SO4 containing 20 mM Tris acetate, pH 7.6, 20 mM sodium chloride, 0.1 mM EDTA, 15 mM β-mercaptoethanol and 1 mM phenylmethylsulfonyl fluoride |
| Preparation Note: | Alpha-actinin ammonium sulfate suspension should be mixed sufficiently and further diluted prior to use. Protein precipitate will be present in solution with high protein concentrations. Prepared using a modification of the procedure of Neidel, J.E. and Cuatrecasas, P., Biochem. Biophys. Res. Commun., 91, 152 (1979). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ~80% α-actinin basis (SDS-PAGE) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |