Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit
SIGMA/A9792 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Rabbit Anti-Chicken IgY (IgG) (whole molecule)−HRP
MDL Number: MFCD00162225
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded, Human tonsil tissue sections were stained with 1:100 Anti-Human IgG (whole molecule) antibody produced in Chicken, followed by 1:500 Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A9792).
ELISA Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody produced in Rabbit (Cat. No. A9792) was serially diluted to detect Chicken IgY (IgG) by direct ELISA.
| antibody form | IgG fraction of antiserum |
| antibody product type | secondary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:30,000 |
| dot blot: 1:160,000 using indirect chemiluminescence system using 5 ng human IgG/dot and chicken anti-human IgG as the primary antibody and Luminol plus enhancer as the substrate. | |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using using human pancreas sections and chicken anti-human insulin as the primary antibody. |
| Application: | Anti-Chicken IgY (IgG) (whole molecule) FITC antibody has been used in immunohistology. |
| Application: | Avian proteins over expressed and purified from E. coli were analyzed by western blot using HRP-conjugated rabbit anti-chicken IgY as the secondary at 1:2000 dilution. |
| Application: | Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody has been used for ELISA. The antibody can also be used for dot blot (1:160,000) and IHC (1:500) assays. |
| Biochem/physiol Actions: | Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications. |
| Biochem/physiol Actions: | IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa. |
| Immunogen: | purified chicken IgY (IgG) |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT. |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Specificity: | Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody binds to chicken IgY. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |