Anti-Androgen Receptor antibody produced in rabbit
SIGMA/A9853 - affinity isolated antibody, buffered aqueous solution
Synonym: Androgen Receptor Antibody; Androgen Receptor Antibody - Anti-Androgen Receptor antibody produced in rabbit; Anti-AR
MDL Number: MFCD01321686
Product Type: Chemical
Immunohistochemistry Formalin fixed, paraffin-embedded Human prostate tissue sections were stained with 20 μg/mL Anti-Androgen Receptor antibody produced in Rabbit (Cat. No. A9853) followed by 15 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunoblotting Rat brain extract was separated on SDS-PAGE and probed with AIA To Androgen Receptor (RB) (Cat. No. A9853). The antibody was developed with HRP Anti-Rabbit IgG (Jackson’s Cat. No. 111-035-045) and ECL substrate. Antibody concentration 1 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 110 kDa (additional band may be observed at 45 kDa) |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20-40 μg/mL using microwave-pretreated human prostate tissue sections |
| immunoprecipitation (IP): 5-10 μg using human MCF7 cell lysate ( 250 μg) | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using whole extract of rat brain | |
| UniProt accession no. | P10275  |
| Application: | Anti-androgen receptor antibody may be used for immunoblotting using whole rat brain extract at a recommended concentration of 1-2 μg/ml. For immunoprecipitaion from 250 μg of human MCF7 cell lysate, a concentration of 5-10 μg may be used. Immunohistochemistry may be performed on paraffin-embedded human prostate tissue sections at a working antibody concentration of 20-40 μg/ml. |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Rabbit anti-androgen receptor (C19) was used in a EMSA (electrophoretic mobility shift assay) which produced a supershift in the molecular weight of a particular DNA sequence indicating binding of the androgen receptor. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Androgens are the male sex hormones that exert biological and transcriptional effects on binding to nuclear androgen receptor (AR). Androgens in humans include androstenedione, testosterone, dihydrotestosterone and dehydroepiandrosterone. Androgen receptor is a ligand inducible nuclear receptor, expressed on brain, bone, skeletal muscles, genital organs, hair follicles and cardiovascular tissue. On binding androgen, the AR is internalized and modulates the transcriptional of target genes. Additionally, AR also exerts extra-nuclear actions wherein it rapidly induces signalling cascades of MAPK and protein kinase C. The regulation by AR results in irreversible sexual differentiation of sex organs during embryogenesis and development of sex-specific characteristics during puberty Anti-Androgen Receptor specifically recognizes the human androgen receptor protein (110 kDa). An additional band may be observed at 45 kDa. The antibody cross-reacts with rat androgen receptor. |
| Immunogen: | evolutionally conserved peptide corresponding to amino acids 1-21 of the human androgen receptor. This sequence is not found in estrogen, progesterone, or glucocorticoid receptors. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Specificity: | The antibody cross-reacts with androgen receptor from rat. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |