7-Amino-4-methylcoumarin
SIGMA/A9891 - Chromophore for substrates
Synonym: Coumarin 120
CAS Number: 26093-31-2
Empirical Formula (Hill Notation): C10H9NO2
Molecular Weight: 175.18
EC Number: 247-454-8
MDL Number: MFCD00006868
Linear Formula: C10H9NO2
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A9891-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A9891-250MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: A9891-500MG
| assay | ≥98% (HPLC) |
| description | chromophore for enzyme substrates |
| fluorescence | λex 365 nm; λem 440 nm in ethanol(lit.) |
| form | powder |
| InChI | 1S/C10H9NO2/c1-6-4-10(12) |
| InChI key | GLNDAGDHSLMOKX-UHFFFAOYSA |
| mp | 223-226 °C (lit.) |
| Quality Level | 200  |
| SMILES string | CC1=CC(=O)Oc2cc(N)ccc12 |
| solubility | acetone: 10 mg/mL, clear, colorless to yellow |
| storage temp. | 2-8°C |
| Application: | 7-Amino-4-methylcoumarin has been used: • to determine cathepsin B like- activity • as a substrate for leucine aminopeptidase (LAP) • in chymotryptic assay • as a standard to detect the activation of caspase-3 during • sanguinarine-induced damage • protection by human DEAD-box DDX3 |
| Application: | Used as matrix for analysis of monosulfated disaccharides and as a co-matrix with 6-aza-2-thiothymidine for sulfated neutral and sialylated tri-and tetrasaccharides. |
| Biochem/physiol Actions: | 7-Amino-4-methylcoumarin is a coumarin derivative, which serves as a fluorescence reference standard to screen protease inhibitors. |
| Other Notes: | Reagent for preparing new fluorogenic AMC-based substrates for cystine aminopeptidase (and other hydrolases); used as reference compound in the enzyme assay. |
| Packaging: | 1 g in poly bottle |
| Packaging: | 250, 500 mg in poly bottle |
| Preparation Note: | Soluble in DMSO (10 mg/mL), DMF, and acetone (10 mg/mL). |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC) |
| mp | 223-226 °C (lit.) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |