Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat
SIGMA/A9917 - affinity isolated antibody, buffered aqueous solution
Synonym: Goat Anti-Mouse IgG (Fab specific)–HRP
MDL Number: MFCD00162623
Product Type: Chemical
Immunohistochemistry Formalin-fixed, paraffin-embedded human appendix sections stained with Monoclonal Anti-Actin, α-Smooth Muscle (Cat. No. A2547) followed by 1:150 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917).
Western Blotting Whole extract of HeLa cells was separated on SDS-PAGE and probed with Anti-β-Actin antibody, Mouse monoclonal, Clone: AC-15 (Cat. No. A1978).The antibody was developed using 1:80,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917) and a chemiluminescent substrate. Lanes 1. 1:80,000 antibody 2. 1:160,000 antibody 3. Negative control: without first antibody
| antibody form | affinity isolated antibody |
| antibody product type | secondary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | peroxidase conjugate |
| form | buffered aqueous solution |
| Quality Level | 300  |
| shipped in | dry ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | direct ELISA: 1:60,000 |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150 | |
| western blot: 1:80,000-1:160,000 |
| Application: | Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody. |
| Application: | Anti-Mouse IgG (Fab specific)-Peroxidase antibody has been used in immunofluorescence, ELISA (enzyme-linked immunosorbent assay) and immunoblotting. Goat Anti-Mouse IgG (Fab specific)-Peroxidase antibody can be used for western blotting assays. The product can also be used for direct ELISA (1:60,000) and IHC (1:150) applications. |
| Application: | Bovine CVEC and human epidermoid carcinoma A431 cell lysates were analyzed by western blot using HRP-conjugated goat anti-mouse IgG Fab specific as the secondary antibody at a 1:3000 dilution. |
| Biochem/physiol Actions: | Immunoglobulin G (IgG) regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG1 regulates complement fixation, opsonization and antibody dependent cell mediated cytotoxicity in mice. IgG participates in hypersensitivity type II and type III. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Immunoglobulin G (IgG) is a glycoprotein antibody. IgG belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. |
| Immunogen: | Purified mouse IgG Fab fragment |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Other Notes: | Antibody adsorbed with human IgG. |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT. |
| Preparation Note: | Adsorbed to reduce background staining with human samples. |
| Preparation Note: | Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978). |
| Specificity: | Specificity of the Anti-Mouse IgG (Fab Specific)- Peroxidase is determined by immunoelectrophoresis (IEP), prior to conjugation. By IEP, single precipitation arcs are observed against normal mouse serum, mouse IgG and the Fab fragment of mouse IgG, while no reaction is observed against the Fc fragment of mouse IgG or human IgG. Binds all mouse IgGs. |
| Storage and Stability: | Store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P280 - P302 + P352 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |