2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

SIGMA/A9941 - tablet, 10 mg substrate per tablet

Synonym: AzBTS-(NH₄)₂; Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)

CAS Number: 30931-67-0
Empirical Formula (Hill Notation): C₁₈H₂₄N₆O₆S₄
Molecular Weight: 548.68
EC Number: 250-396-6
MDL Number: MFCD00010404
Linear Formula: C₁₈H₂₄N₆O₆S₄
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-A9941-5TAB	5 tabs	$58.00 1/EA	Add To Favorites
45-A9941-50TAB	50 tabs	$223.00 1/EA	Add To Favorites
45-A9941-100TAB	100 tabs	$388.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

form	tablet
InChI	1S/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18-;;
InChI key	OHDRQQURAXLVGJ-AXMZSLBLSA-N
Quality Level	200
SMILES string	[NH4+].[NH4+].CCN1C(Sc2cc(ccc12)S([O-])(=O)=O)=NN=C3/Sc4cc(ccc4N3CC)S([O-])(=O)=O
solubility	deionized water: 100 mL
storage temp.	room temp

Application:	• 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS, has been used as a substrate in ELISA (enzyme-linked immunosorbent assay).† • ABTS is also used to measure glucose concentrations in different solutions including blood serum. • The food industry uses ABTS to measure the antioxidant capacities within different foods. • Since it is less readily oxidized than TMB and OPD substrates, ABTS is less sensitive for ELISA applications. Thus ABTS has some advantageous over TMB and OPD in cases of large background results • It is recommended for ELISA (microwell) procedures, not recommended for membrane applications.
Application:	2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is green in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
General description:	ABTS mainly used in ELISA (enzyme-linked immunosorbent assay) procedures. ABTS, 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), is a chromogenic substrate used: • For a reaction with laccase or bilirubin oxidase • For a reaction with peroxidase enzymes: (like HRP for example). ABTS in combination with hydrogen peroxide A peroxidase reaction of ABTS in the presence of hydrogen peroxide produces a green soluble end product which can be read spectrophotometrically at 405nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). ABTS, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is light sensitive, and water soluble chemical compound
Reconstitution:	Dissolve 1 tablet in 100 ml of 0.05 M phosphate-citrate buffer, pH 5.0 (Tablets available as Sigma Product No. P4809). Add 25 μl of fresh 30% hydrogen peroxide

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	room temp
UNSPSC	12352204

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