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Ammonia Assay Kit

SIGMA/AA0100 - sufficient for 100 assays

Synonym: Ammonia Quantitative Test Kit

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-AA0100-1KT 1 kit
 $382.00
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Quality Level 200 
storage temp. 2-8°C
usage sufficient for 100 assays
Application: Ammonia concentration was measured in serum and urine using the Sigma Ammonia Assay Kit in a study assessing expression of renal ammonia transporters in response to chromic metabolic acidosis.
Application: This kit is for the quantitative, enzymatic determination of ammonia in food and biological samples. Ammonia reacts with α-ketoglutaric acid (KGA) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) in the presence of L-glutamate dehydrogenase (GlDH) to form L-glutamate and oxidized nicotinamide adenine dinucleotide phosphate (NADP+). The decrease in absorbance at 340 nm, due to the oxidation of NADPH, is proportional to the ammonia concentration. L-Glutamate dehydrogenase reacts specifically with ammonia. The Ammonia Assay Kit may be used to determine ammonia concentrations in the range of 0.2-15 μg/ml.
Storage Temp. 2-8°C
UNSPSC 12352202
Components Ammonia Assay Reagent 10 vial(s); L-Glutamate Dehydrogenase for Ammonia Assay 1 mL/vial; Ammonia Standard Solution 1 mL/vial

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