Alkaline Phosphatase Blue Membrane Substrate Solution
SIGMA/AB0300 - sufficient for 400 mL working substrate
Product Type: Chemical
| form | solution |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| usage | sufficient for 400 mL working substrate |
| Application: | Alkaline Phosphatase Blue Membrane Substrate Solution has been used: • to analyse direct alkaline phosphatase (AP) activity • to perform alkaline phosphatase assays with colorimetric system • to analyse direct alkaline phosphatase (AP) activity of the induced pluripotent stem cells (iPSC) line LND554SV.3 |
| Application: | Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue and the Nitro BT reagent is yellow. The two-component mixture develops a bluish-purple product with alkaline phosphatase in membrane-type assays. This substrate is not recommended for ELISA assays. |
| General description: | Alkaline phosphatase, a metalloenzyme is a member of ectonucleotidases family. It is present in bile ducts, bone, intestine, placenta and tumors. |
| Legal Information: | BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | A 2 component kit sufficient for preparing 400 mL of working substrate. |
| Physical form: | Supplied as a two-component buffered alkaline phosphatase substrate containing bromochloroindolyl phosphate (BCIP®) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT) |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352204 |