Monoclonal Anti-FUS antibody produced in mouse
SIGMA/AMAB90549 - Prestige Antibodies® Powered by Atlas Antibodies, clone CL0190, purified immunoglobulin, buffered aqueous glycerol solution
Synonym: ALS6; FUS1; TLS; hnRNP-P2
Product Type: Product-on-demand
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human cerebral cortex and liver tissues using AMAB90549 antibody. Corresponding FUS RNA-seq data are presented for the same tissues.
Enhanced Validation-RNAi Knockdown Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probes 1 and 2, using Anti-FUS antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry Immunohistochemical staining of human endometrium shows strong nuclear positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry Immunohistochemical staining of human liver shows only very weak nuclear positivity in hepatocytes.
Immunofluorescence Immunofluorescence staining in HeLa cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence Immunofluorescence staining in A431 cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence Immunofluorescence staining in MCF7 cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence Immunofluorescence staining in U2OS cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Immunofluorescence Immunofluorescence staining in U251 cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Western Blotting Lane 1: Marker [kDa] Lane 2: Human cell line HeLa Lane 3: Human cell line A-431 Lane 4: Human cell line MCF-7 Lane 5: Human cell line U2-OS Lane 6: Human cell line Hep-G2
Western Blotting Lane 1: Marker [kDa] Lane 2: Human cell line HeLa cytoplasmic fraction Lane 3: Human cell line HeLa membrane fraction Lane 4: Human cell line HeLa nuclear fraction Lane 5: Human cell line HeLa chromatin fraction Lane 6: Human cell line HeLa cytoskeletal fraction
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CL0190, monoclonal |
| conjugate | unconjugated |
| enhanced validation | RNAi knockdown orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| Ensembl | human accession no. | ENSG00000089280  |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1 μg/mL |
| immunofluorescence: 2-10 μg/mL | |
| immunohistochemistry: 1:2500- 1:5000 | |
| UniProt accession no. | P35637 |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| Immunogen: | fused in sarcoma recombinant protein epitope signature tag (PrEST) Sequence SSQSSYGQQSSYPGYGQQPAPSSTS Epitope Binds to an epitope located within the peptide sequence YGGQQQSYGQ as determined by overlapping synthetic peptides. |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Linkage: | Corresponding Antigen APREST86697 |
| Physical form: | Phospate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |