Monoclonal Anti-PCM1 antibody produced in mouse
SIGMA/AMAB90565 - Prestige Antibodies® Powered by Atlas Antibodies, clone CL0206, purified immunoglobulin, buffered aqueous glycerol solution
Synonym: PTC4
Product Type: Product-on-demand
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human testis and pancreas tissues using AMAB90565 antibody. Corresponding PCM1 RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunohistochemical staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry Immunohistochemical staining of human fallopian tube shows moderate to strong positivity in the apical cytoplasm of glandular cells.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows weak cytoplasmic positivity in neurons.
Immunohistochemistry Immunohistochemical staining of human pancreas shows very weak cytoplasmic positivity in exocrine glandular cells as expected.
Western Blotting Lane 1: Marker [kDa] Lane 2: Human cell line U-251
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CL0206, monoclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| Ensembl | human accession no. | ENSG00000078674  |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1 μg/mL |
| immunohistochemistry: 1:200- 1:500 | |
| UniProt accession no. | Q15154 |
| Biochem/physiol Actions: | Pericentriolar material 1 (PCM1) plays a major role in the development of cell cycle. It maintains the centrosome integrity and controls the microtubule cytoskeleton. PCM1 plays a vital role in the progression of the nervous system and neuronal activity. This protein participates in the enrolment of GABARAP (γ-aminobutyric acid receptor-associated protein) to the pericentriolar material. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Pericentriolar material 1 (PCM1) gene codes for a 228 kDa protein. It has various coiled-coil domains in its amino-terminal. It is located in the cytoplasmic granules and is termed as centriolar satellites. PCM1 is located on human chromosome 8p22. |
| Immunogen: | pericentriolar material 1 recombinant protein epitope signature tag (PrEST) Sequence TIYSEVATLISQNESRPHFLIELFH Epitope Binds to an epitope located within the peptide sequence RQRALYALQD as determined by overlapping synthetic peptides. |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Linkage: | Corresponding Antigen APREST76188 |
| Physical form: | Phospate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |