Monoclonal Anti-WWTR1 antibody produced in mouse
SIGMA/AMAB90730 - Prestige Antibodies® Powered by Atlas Antibodies, clone CL0371, purified immunoglobulin, buffered aqueous glycerol solution
Synonym: DKFZp586I1419; TAZ
Product Type: Product-on-demand
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human placenta and lymph node tissues using AMAB90730 antibody. Corresponding WWTR1 RNA-seq data are presented for the same tissues.
Enhanced Validation-RNAi Knockdown Western blot analysis in EFO-21 cells transfected with control siRNA, target specific siRNA probes 1 and 2, using Anti-WWTR1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Immunohistochemistry Immunohistochemical staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry Immunohistochemical staining of human placenta shows strong nuclear and some cytoplasmic positivity in endothelial cells.
Immunohistochemistry Immunohistochemical staining of human kidney shows strong nuclear and moderate cytoplasmic positivity in cells in tubules and in glomeruli.
Immunohistochemistry Immunohistochemical staining of human lymph node shows no nuclear positivity in lymphoid cells as expected.
Immunofluorescence Immunofluorescence staining in SKMEL cell line with Anti-WWTR1 monoclonal antibody, showing spotty nuclear staining in green. Microtubule probes are visualized in red (where available).
Western Blotting Lane 1: Marker [kDa] Lane 2: Human cell line U-251 MG
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CL0371, monoclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq RNAi knockdown Learn more about Antibody Enhanced Validation  |
| Ensembl | human accession no. | ENSG00000018408  |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1 μg/mL |
| immunofluorescence: 2-10 μg/mL | |
| immunohistochemistry: 1:50- 1:200 | |
| UniProt accession no. | Q9GZV5 |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | WWTR1(WW domain containing transcription regulator 1)/TAZ (transcriptional co-activator with PDZ-binding motif) stimulates the multiplication of glioma cell and represses the apoptosis of glioma cells. TEADs are important interacting transcription factors of TAZ, which serve as effectors to promote cell proliferation, migration and epithelial-mesenchymal transition. TAZ participates in mesenchymal stem cell differentiation. It plays a vital role in mechanotransduction. Overexpression of TAZ leads to increased cell proliferation, epithelial-mesenchymal transition (EMT), cell migration and transformation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | WWTR1 (WW domain containing transcription regulator 1) is also known as TAZ (transcriptional co-activator with PDZ-binding motif). It is a transcriptional coactivator with a N-terminal TEAD-binding motif and without a DNA-binding motif. TAZ is a 14-3-3 binding protein that is expressed at high level in thyroid, kidney, heart, placenta and lung. It is present in cytoplasm and nucleus. The WWTR1 gene is located on human chromosome 3q25.1. |
| Immunogen: | Recombinant protein corresponding to WW domain containing transcription regulator 1. Sequence MNPKPSSWRKKILPESFFKEPDSGS Epitope Binds to an epitope located within the peptide sequence SSWRKKILPESFFKE as determined by overlapping synthetic peptides. |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Linkage: | Corresponding Antigen APREST70841 |
| Physical form: | Phospate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |