Monoclonal Anti-HNF1B antibody produced in mouse
SIGMA/AMAB90733 - Prestige Antibodies® Powered by Atlas Antibodies, clone CL0374, purified immunoglobulin, buffered aqueous glycerol solution
Synonym: Anti-HNF1beta; Anti-LFB3; Anti-MODY5; Anti-TCF2; Anti-VHNF1
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human kidney and tonsil tissues using AMAB90733 antibody. Corresponding HNF1B RNA-seq data are presented for the same tissues.
Enhanced Validation-Orthogonal RNAseq Confirmation Western blot analysis in human cell lines Caco-2 and A-431 using Anti-HNF1B antibody. Corresponding HNF1B RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.
Immunohistochemistry Immunohistochemical staining of human kidney shows strong nuclear positivity in cells in tubules.
Immunohistochemistry Immunohistochemical staining of human colon shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human renal cancer shows strong nuclear positivity in tumor cells.
Immunohistochemistry Immunohistochemical staining of human colorectal cancer shows moderate nuclear positivity in tumor cells.
Immunohistochemistry Immunohistochemical staining of human tonsil shows no positivity in lymphoid cells as expected.
Immunofluorescence Immunofluorescence staining in A549 cell line with Anti-HNF1B monoclonal antibody, showing spotty nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available).
Western Blotting Lane 1: Marker [kDa] Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: HNF1B Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400162)
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CL0374, monoclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | KEVLVQALEELLPSPNFGVKLETLP |
| isotype | IgG1 |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1 μg/mL |
| immunofluorescence: 2-10 μg/mL | |
| immunohistochemistry: 1:500- 1:1000 | |
| UniProt accession no. | P35680  |
| Application: | All Prestige Antibodies® Powered by Atlas Antibodies is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| Immunogen: | HNF1 homeobox B |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Phospate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |