Monoclonal Anti-RHOT1 antibody produced in mouse
SIGMA/AMAB90854 - Prestige Antibodies® Powered by Atlas Antibodies, clone CL1095, purified immunoglobulin, buffered aqueous glycerol solution
Synonym: ARHT1; FLJ11040; MIRO-1
Product Type: Product-on-demand
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells, as well as positivity in neuropil.
Immunohistochemistry Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.
Immunohistochemistry Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows only very weak cytoplasmic positivity in striated muscle fibers.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CL1095, monoclonal |
| conjugate | unconjugated |
| Ensembl | human accession no. | ENSG00000126858   |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:200- 1:500 |
| UniProt accession no. | Q8IXI2 |
| Application: | Monoclonal Anti-RHOT1 antibody has been used in Immunoprecipitation. |
| Biochem/physiol Actions: | Miro1/RHOT1 (ras homolog family member T1) regulates mitochondrial trafficking and distribution. The Rho GTPase Miro-1 plays a crucial role in the modulation of mitochondrial morphogenesis and acts as a calcium-dependent sensor for the control of mitochondrial motility. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Miro1/RHOT1 (ras homolog family member T1) is an outer mitochondrial membrane (OMM) protein. It has a transmembrane domain, two GTPase domains and two Ca2+-sensing EF-hand domains. RHOT1 is located on human chromosome 17q11. |
| Immunogen: | ras homolog family member T1, recombinant protein epitope signature tag (PrEST) Sequence THIVDYSEAEQSDEQLHQEISQANV Epitope Binds to an epitope located within the peptide sequence ERTDKDSRLP as determined by overlapping synthetic peptides. |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST71907 |
| Physical form: | Phospate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |