Monoclonal Anti-ITGAM antibody produced in mouse
SIGMA/AMAB90911 - Prestige Antibodies® Powered by Atlas Antibodies, clone CL1719, purified immunoglobulin, buffered aqueous glycerol solution
Synonym: Anti-CD11B; Anti-CR3A; Anti-MAC-1
Product Type: Product-on-demand
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human tonsil and skeletal muscle tissues using AMAB90911 antibody. Corresponding ITGAM RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunohistochemical staining of human tonsil shows strong membranous positivity in germinal center cells.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows moderate membranous positivity in microglia.
Immunohistochemistry Immunohistochemical staining of human colon shows moderate membranous positivity in lymphoid cells.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.
Western Blotting Lane 1: Marker [kDa] Lane 2: Human tonsil
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CL1719, monoclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | LNFTASENTSRVMQHQYQVSNLGQR |
| isotype | IgG1 |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1 μg/mL |
| immunohistochemistry: 1:1000- 1:2500 | |
| UniProt accession no. | P11215  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Application: | Monoclonal Anti-ITGAM Prestige Antibodies® Powered by Atlas Antibodies is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using protein array and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/ Prestige . |
| Biochem/physiol Actions: | Integrin α M (ITGAM) is involved in cell activation, chemotaxis, cytotoxicity and phagocytosis. It modulates the interaction of leukemic cells with microenvironment by binding inactivated complement component 3b (iC3b), intercellular adhesion molecule (ICAM), fibrinogen, β-glukanes, coagulation factor X etc. ITGAM is considered as a marker for myeloid-derived suppressor cells that participates in restraining antitumor immunity of the host and inducing the enlargement and drug-resistance of hematological malignant cells. D11b integrin contributes to ICAM-1-dependent AIDP patient mononuclear leukocyte adhesion. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Integrin α M (ITGAM) is also called as cluster of differentiation 11b (CD11b), intercellular adhesion molecule-1 (ICAM-1), complement component 3 receptor α chain (CR3α), macrophage-1 antigen α subunit or macrophage receptor 1 α subunit (MAC1α). It is expressed on monocytes/macrophages, natural killer cells, neutrophils and a subset of B-and T-lymphocytes. ITGAM gene is located on human chromosome 16p11. |
| Immunogen: | integrin, alpha M (complement component 3 receptor 3 subunit) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST83070 |
| Physical form: | Phospate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |