Monoclonal Anti-NEFM antibody produced in mouse
SIGMA/AMAB91030 - Prestige Antibodies® Powered by Atlas Antibodies, clone CL2705, purified immunoglobulin, buffered aqueous glycerol solution
Synonym: NEF3; NF-M; NFM
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of rat brainstem shows strong immunoreactivity in mesencephalic trigeminal nucleus neurons.
Immunohistochemistry Immunohistochemical staining of mouse cerebral cortex shows strong immunoreactivity in neurofilaments.
Immunohistochemistry Immunohistochemical staining of human cerebellum shows strong immunoreactivity in Purkinje cells and neuronal processes.
Immunohistochemistry Immunohistochemical staining of rat cerebellum shows strong immunoreactivity in neuronal processes.
Immunohistochemistry Immunohistochemical staining of mouse trigeminal ganglion shows strong positivity in primary sensory neurons.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows strong positivity in neural fibers.
Immunohistochemistry Immunohistochemical staining of human liver shows absence of immunoreactivity (negative control).
Western Blotting Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human Cerebral Cortex tissue
Western Blotting Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Mouse Cerebral Cortex tissue
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | CL2705, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | YIEKVHYLEQQNKEIEAEIQALRQK |
| isotype | IgG2b |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 1 μg/mL |
| immunohistochemistry: 1:5000- 1:10000 | |
| UniProt accession no. | P07197  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | Neurofilament medium (NEFM) is essential for radial growth of axon which includes the diameter of the axon as well as the myelination. The C-terminus of NFEM gene has 7 repeats of KSP (lysine-serine-proline) domain and phosphorylation of which is crucial for regulating the radial growth of axon. NEFM is related to several cancers especially breast cancers in which NEFM gene silencing by methylation leads to disease progression. Mutations in NEFM results in decreased diameter of axon and hence deficiency of neurons. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Neurofilament medium (NEFM) gene encodes neurofilament medium polypeptide which constitutes to cytoskeleton of mature neurons. The gene in human chromosome is localized on 8p21. |
| Immunogen: | neurofilament, medium polypeptide |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |