Monoclonal Anti-MAP2 antibody produced in mouse
SIGMA/AMAB91375 - Prestige Antibodies® Powered by Atlas Antibodies, clone CL5420, purified immunoglobulin, buffered aqueous glycerol solution
Synonym: MAP2A; MAP2B; MAP2C
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows cytoplasmic immunoreactivity in neurons, as well as positivity in neuropil.
Immunohistochemistry Immunohistochemical staining of human cerebellum shows positivity in a subset of neurons.
Immunohistochemistry Immunohistochemical staining of human pancreas shows cytoplasmic immunoreactivity in pancreatic islets.
Immunohistochemistry Immunohistochemical staining of human tonsil shows absence of positivity in lymphoid cells as expected (negative control).
Immunofluorescence Immunofluorescence staining of SH-SY5Y cells using the Anti-MAP2 monoclonal antibody, showing specific staining in the cytosol and in microtubules in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Western Blotting Western blot analysis in human cell line SH-SY5Y.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | CL5420, monoclonal |
| conjugate | unconjugated |
| Ensembl | human accession no. | ENSG00000078018   |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 2-10 μg/mL |
| immunohistochemistry: 1:1000-1:2500 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P11137 |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| Immunogen: | Recombinant protein corresponding to microtubule-associated protein 2 Sequence EAKAPHWTSAPLTEASAHSHPPEIK Epitope Binds to an epitope located within the peptide sequence PPEIKDQGGAGEGLV as determined by overlapping synthetic peptides. |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |