Alkaline Phosphatase Detection Kit, Fluorescence
SIGMA/APF
EC Number: 245-325-0
Product Type: Chemical
Alkaline Phosphatase activity by dilution
| grade | for molecular biology |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | microbe id | specific enzyme detection: suitable |
| usage | kit sufficient for 300 reactions (200 μl reactions) |
| Application: | Alkaline Phosphatase Detection Kit, Fluorescence has been used to determine alkaline phosphatase (ALP) activity. |
| Application: | Suitable for measuring the alkaline phosphatase (AP) or secreted alkaline phosphatase (SEAP)reporter gene activity. The Alkaline Phosphatase Kit can be used to determine the strength of promoters and enhancers, define the role of transcription factors, assess transfection efficiency, and measure the success of molecular cloning attempts. Also suitable for detection of AP activity in pluripotent stem cells, including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). |
| Biochem/physiol Actions: | Alkaline phosphatase (ALP) helps in hydrolyses and transphosphorylation of several phosphoric acid monoesters. It modulates various intracellular processes, that are associated with cell cycle, apoptosis, growth and signal transduction pathways. |
| Components: | The Alkaline Phosphatase Kit contains: 50mL Fluorescent Assay Buffer ( B6558) 6 mL Dilution Buffer (B6433) 50 μl Alkaline Phosphatase Control Enzyme, 0.1 mg/mL (C9361) 2 x 1 mg 4-Methylumbelliferyl phosphate disodium (M8168) |
| Features and Benefits: | • Up to 100x more sensitive than colorimetric assays • SEAP can be measured without sample destrutcion • Rapid and reproducible results • Safe and easy-to-use |
| General description: | The Alkaline Phosphatase (AP) Kit uses a fluorometric assay to detect AP activity. The kit capability is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis. |
| General description: | The gene for alkaline phosphatase (AP) commonly serves as a reporter gene and is used to determine the strength of promoters and enhancers, define the role of transcription factors, assess transfection efficiency and measure the success of molecular cloning attempts. The alkaline phosphatase (AP) kit uses a fluorometric assay to detect AP activity. The kit capability is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |