Annexin V-Cy3™ Apoptosis Detection Kit
SIGMA/APOAC
Synonym: Red fluorescent Annexin V apoptosis assay
Product Type: Chemical
application(s) | cell analysis detection |
detection method | fluorometric |
packaging | pkg of 1 kit |
Quality Level | 200 |
shipped in | wet ice |
storage temp. | 2-8°C |
technique(s) | flow cytometry: suitable |
usage | sufficient for 200 tests |
Features and Benefits: | Detects apoptosis earlier in the process than DNA-based assays such as TUNEL • Rapid labeling of cells. Cell staining takes only 10 minutes. • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study. • 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells. |
Legal Information: | Cy3 is a trademark of Cytiva |
Principle: | Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive). |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | 2-8°C |
UNSPSC | 12352207 |
Components | 6-Carboxyfluorescein diacetate 10 mg; Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg |