HIV Protease Activity Detection Kit
SIGMA/APPA014
Synonym: Cut-N-Glow HIV Protease Detection Kit
Product Type: Chemical
form | solution |
Quality Level | 200 |
shipped in | dry ice |
storage temp. | −20°C |
Application: | This kit is specific for the HIV Protease, a retroviral aspartyl protease that is critical to viral life-cycle. The Cut-N-Glow approach involves the introduction of a structural distortion into one of the complementary fragments (GFP 11), through the use of a conditionally stable tether, which serves to constrain the N and C termini of GFP 11 closely in space, thereby, diminishing the mutual affinity of the two fragments and blocking protein self-assembly until the tether is cleaved. The distortion can be reversed upon site-specific proteolysis of the tether, resulting in GFP 11 fragment assembly with GFP 1-10, generating reconstituted, functional GFP. Depending on the tether, the chimeric GFP can serve as a substrate for proteases from the three major classes: serine, cysteine, and aspartic acid. |
Suitability: | This kit contains sufficient reagents for one 96 well plate (96 tests) |
RIDADR | NONH for all modes of transport |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | −20°C |
UNSPSC | 12352200 |