Anti-SMAD3 antibody produced in rabbit
SIGMA/AV100621 - IgG fraction of antiserum
Synonym: Anti-SMAD family member 3
Product Type: Chemical
Immunohistochemistry Anti-SMAD3: Cat. No. AV100621: Immunohistochemistry of SMAD3 in Human Pancreas tissue with SMAD3 antibody at 4-8 μg/mL.
Immunohistochemistry Immunohistochemistry of SMAD3 in Human Urinary Bladder with SMAD3 antibody at 4-8 μg/mL
Immunoblotting Cell Type: liver (Cat. No. AV100621). Lanes 1. Antibody dilution (concentration) 2.0 μg/mL in cell type liver
Western Blotting Western Blot of SMAD3 in Mouse Testis with SMAD3 antibody at 1 μg/mL
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 48 kDa |
| NCBI accession no. | NP_005893   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | dog, rat, human, mouse, guinea pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P84022 |
| Application: | Anti-SMAD3 antibody produced in rabbit is suitable for western blotting at a concentration of 2 μg/ml. For immunohistochemistry of paraffin-embedded tissue sections, a concentration of 4-8 μg/ml is suitable. |
| Biochem/physiol Actions: | Smad3 is phosphorylated by TGF-b, heterodimerizes with Smad4 and enters the nucleus. The heterodimer binds within the promoter of plasminogen activator inhibitor-1 (PAI-1) and induces its expression. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The members of Smad family of proteins are essential intracellular mediators of TGF-β signaling. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human SMAD3 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: FTPPIVKRLLGWKKGEQNGQEEKWC |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |