Anti-CBX4 antibody produced in rabbit
SIGMA/AV30002 - affinity isolated antibody
Synonym: Anti-Chromobox homolog 4 (Pc class homolog, Drosophila)
Product Type: Chemical
Immunohistochemistry Anti-CBX4: Cat. No. AV30002: Immunohistochemistry of CBX4 in Human Kidney tissue with CBX4 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: Jurkat (Cat. No. AV30002). Lanes 1. Antibody dilution (concentration) 0.5 μg/mL in cell type Jurkat
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 61 kDa |
| NCBI accession no. | NP_003646   |
| shipped in | wet ice |
| species reactivity | rat, guinea pig, bovine, rabbit, dog, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | O00257-2 |
| Application: | Anti-CBX4 antibody produced in rabbit is suitable for western blotting at a concentration of 0.5 μg/ml. For immunohistochemistry of paraffin-embedded tissue sections, a concentration of 4-8 μg/ml is suitable. |
| Biochem/physiol Actions: | CBX4 belongs to the Polycomb group of proteins that are involved in determining stem cell pluripotency, identity and epigenetic gene silencing. CBX4 exhibits E3 sumo ligase activity that is directly involved in DNA damage response pathway. It interacts with p63 and regulates the transcription of genes that maintain the stemness of epithelial progenitors. CBX4 has a non-redundant role in T-cell proliferation and thymic organogenesis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human CBX4 |
| Other Notes: | Synthetic peptide located within the following region: LLIAFQNRERQEQLMGYRKRGPKPK |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |