Anti-GLIS2 antibody produced in rabbit
SIGMA/AV30037 - affinity isolated antibody
Synonym: Anti-GLIS family zinc finger 2
Product Type: Chemical
Immunohistochemistry Anti-GLIS2: Cat. No. AV30037: Immunohistochemistry of GLIS2 in Human Bile Duct tissue with GLIS2 antibody at 10 μg/mL.
Immunoblotting Cell Type: HepG2 (Cat. No. AV30037). Lanes 1. Antibody dilution (concentration) 0.5 μg/mL in cell type HepG2
Western Blotting Western Blot of GLIS2 in Human Ovary Tumor with GLIS2 antibody at 1 μg/mL
Western Blotting Western Blot of GLIS2 in Human 786-0 Whole Cell with GLIS2 antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 56 kDa |
| NCBI accession no. | NP_115964   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | dog, human, horse, bovine, rat, guinea pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q9BZE0 |
| Application: | Rabbit polyclonal anti-GLIS2 antibody is used to tag GLIS family zinc finger 2 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of GLIS family zinc finger 2 in kidney development and maintenance. Anti-GLIS2 antibody produced in rabbit is suitable for western blotting at a concentration of 0.5 μg/ml. |
| Biochem/physiol Actions: | Members of the Kruppel-like zinc finger protein family, such as GLIS2, function as activators and/or repressors of gene transcription. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | GLIS family zinc finger 2 (GLIS2), a Krüppel-like zinc finger protein family member with transactivation and repressor functions, is involved in kidney development, the maintenance of normal renal function and neurogenesis. Glis2 interacts with β-catenin and may function as a negative modulator of β-catenin/TCF-mediated transcription. Defective GLIS2 has been linked to the development of nephronophthisis. |
| General description: | Rabbit polyclonal anti-GLIS2 antibody reacts with canine, human, chicken, rat, bovine, and mouse GLIS family zinc finger 2 transcription factors. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human GLIS2 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: QDLVDHVNDYHVKPEKDAGYCCHWE |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |