Anti-E2F4 antibody produced in rabbit
SIGMA/AV31175 - affinity isolated antibody
Synonym: Anti-E2F transcription factor 4, p107/p130-binding
Product Type: Chemical
Immunohistochemistry Anti-E2F4: Cat. No. AV31175: Immunohistochemistry of E2F4 in Human Stomach tissue with E2F4 antibody at 4-8 μg/mL.
Immunohistochemistry Anti-E2F4: Cat. No. AV31175: Immunohistochemistry of E2F4 in Human Stomach tissue with E2F4 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: HepG2 (Cat. No. AV31175). Lanes 1. Antibody dilution (concentration) 2.0 μg/mL in cell type HepG2
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 44 kDa |
| NCBI accession no. | NP_001941   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rabbit, guinea pig, rat, bovine, dog, human, horse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 4-8 μg/mL (Paraffin-embedded Tissue) |
| western blot: suitable | |
| UniProt accession no. | Q16254 |
| Application: | Rabbit Anti-E2F4 antibody can be used for western blot (2.0μg/ml) and IHC (4-8μg/ml) applications. |
| Biochem/physiol Actions: | The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins. It is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It also plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human E2F4 |
| Other Notes: | Synthetic peptide located within the following region: SSELLEELMSSEVFAPLLRLSPPPG |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |