Anti-ISGF3G antibody produced in rabbit
SIGMA/AV31200 - affinity isolated antibody
Synonym: Anti-IRF-9; Anti-ISGF3; Anti-ISGF3G; Anti-p48
Product Type: Chemical
Immunohistochemistry Anti-ISGF3G: Cat. No. AV31200: Immunohistochemistry of ISGF3G in Human Liver tissue with ISGF3G antibody at 4-8 μg/mL.
Immunoblotting Cell Type: Jurkat (Cat. No. AV31200). Lanes 1. Antibody dilution (concentration) 2.5 μg/mL in cell type Jurkat
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 44 kDa |
| NCBI accession no. | NP_006075   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rabbit, human, rat, bovine, mouse, guinea pig, dog, horse |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q00978 |
| Application: | Rabbit Anti-ISGF3G antibody can be used for western blotting applications at 2.5μg/ml. |
| Application: | Rabbit polyclonal anti-ISGF3G antibody is used to tag interferon regulatory factor 9/ISGF3 gamma for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of interferon regulatory factor 9/ISGF3 gamma in the mediation of interferon-α and β (type I IFNs) signaling. |
| Biochem/physiol Actions: | ISGF3G functions to recruit RNA polymerase II to the promoter of interferon-stimulated genes and requires histone deacetylases. Defects in ISGF3 can cause resistance to IFN-.(2a) treatment. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Interferon regulatory factor 9 (ISGF3G, IRF-9, p48) is a DNA-binding component of the heterotrimeric transcription factor ISGF3 which is activated by interferon-α and β (type I IFNs). The other two components of ISGF3 are STAT1 and STAT2. ISGF3 gamma or complexes containing ISGF3 gamma are involved in IRF-1-independent pathways mediating IFN gene regulation. ISGF3 plays a primary role in the transmission of a signal from the cell surface to the nucleus via regulatory factors p84/91 and p113. |
| General description: | Rabbit polyclonal anti-ISGF3G antibody reacts with pig, bovine, human, mouse, rat, and zebrafish interferon regulatory factor 9/ISGF3 gamma proteins. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human ISGF3G |
| Other Notes: | Synthetic peptide located within the following region: PWKHAGKQDFREDQDAAFFKAWAIF |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |