Anti-NFYA antibody produced in rabbit
SIGMA/AV31263 - IgG fraction of antiserum
Synonym: Anti-Nuclear transcription factor Y, α
Product Type: Chemical
Immunofluorescence Tissue: liver (Cat. No. AV31263). Specification 1. Antibody dilution (concentration) 4-8 μg/mL
Immunoblotting Cell Type: Jurkat (Cat. No. AV31263). Lanes 1. Antibody dilution (concentration) 5.0-8.0 μg/mL in cell type Jurkat
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 37 kDa |
| NCBI accession no. | NP_068351   |
| shipped in | wet ice |
| species reactivity | human, mouse, horse, bovine, rat, rabbit, guinea pig, dog |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P23511 |
| Application: | Rabbit Anti-NFYA antibody can be used for immunohistochemistry (4-8μg/ml using paraffin-embedded tissues) and western blot (5-8μg/ml) applications. |
| Biochem/physiol Actions: | NFYA is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | NFYA is a nuclear transcription factor that forms a regulatory subunit of the trimeric complex of NF-Y that associates with CCAAT motifs in cell cycle progression genes. Hence, this gene has been implicated in cell proliferation. Furthermore, NFYA can also facilitate the self-renewal of hematopoietic stem cells (HSCs). Rabbit Anti-NFYA antibody recognizes canine, chicken, human, mouse, rat, and bovine NFYA. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human NFYA |
| Other Notes: | Synthetic peptide located within the following region: YLHESRHRHAMARKRGEGGRFFSPK |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |