Anti-ATF1 (AB2) antibody produced in rabbit
SIGMA/AV31366 - affinity isolated antibody
Synonym: Anti-Activating transcription factor 1; Anti-EWS-ATF1; Anti-FUS/ATF-1; Anti-TREB36
Product Type: Chemical
Immunohistochemistry Anti-ATF1 (ab2): Cat. No. AV31366: Immunohistochemistry of ATF1 in Thymus tissue with ATF1 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: 721_B (Cat. No. AV31366). Lanes 1. Antibody dilution (concentration) 0.5 μg/mL in cell type 721_B
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 29 kDa |
| NCBI accession no. | NP_005162   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rabbit, horse, dog, rat, guinea pig, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P18846 |
| Application: | Rabbit Anti-ATF1 (AB2) antibody can be used for western blotting (0.5μg/ml) applications. |
| Biochem/physiol Actions: | ATF1 binds the cAMP response element (CRE) (consensus: 5′-GTGACGT [AC] [AG]-3′), a sequence present in many viral and cellular promoters. ATF1 binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | ATF1 is a member of the AMP response element-binding protein/activating transcription factor (CREB/ATF) that associates with BRCA1. ATF1 target genes have been implicated in mediating transcriptional response to DNA damage. Moreover, studies have reported that phosphorylation of ATF1 is needed for growth factor-induced expression of c-jun. Rabbit Anti-ATF1 (AB2) antibody recognizes rat, mouse, zebrafish, bovine, and human ATF1. |
| Immunogen: | Synthetic peptide directed towards the middle region of human ATF1 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: YQIRTTPSATSLPQTVVMTSPVTLT |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |