Anti-EN2 antibody produced in rabbit
SIGMA/AV31375 - affinity isolated antibody
Synonym: Anti-AUTS1; Anti-AUTS10; Anti-Engrailed homeobox 2
MDL Number: MFCD03096780
Product Type: Chemical
Immunohistochemistry Anti-EN2: Cat. No. AV31375: Immunohistochemistry of EN2 in Human Kidney tissue with EN2 antibody at 4-8 μg/mL.
Immunohistochemistry Immunohistochemistry of EN2 in Human Kidney with EN2 antibody at 4-8 μg/mL
Immunoblotting Cell Type: fetal liver (Cat. No. AV31375). Lanes 1. Antibody dilution (concentration) 0.5 μg/mL in cell type fetal liver
Western Blotting Western Blot of EN2 in Human Jurkat with EN2 antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 34 kDa |
| NCBI accession no. | NP_001418   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | dog, mouse, guinea pig, horse, human, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P19622 |
| Application: | Rabbit Anti-EN2 antibody is suitable for use in western blot (0.5μg/ml) assays. |
| Application: | Rabbit polyclonal anti-ENS antibody is used to tag engrailed homeobox 2 transcription factor for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of engrailed homeobox 2 transcription factor in brain development and skeletal muscle differentiation. |
| Biochem/physiol Actions: | Homeobox-containing genes are thought to have a role in controlling development. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Engrailed homeodomain-containing transcription factors play crucial roles in brain development across many species, including the determination of the hindbrain/midbrain border, cerebellar patterning and aiding in neuronal axon guidance. Engrailed-2 (En-2) gene is expressed across the mesencephalon/metencephal |
| General description: | Rabbit polyclonal anti-ENS antibody reacts with chicken, zebrafish, human, mouse, and rat engrailed homeobox 2 transcription factors. |
| Immunogen: | Synthetic peptide directed towards the C-terminal region of Human EN2 |
| Other Notes: | Synthetic peptide located within the following region: NESQIKIWFQNKRAKIKKATGNKNT |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |