Anti-MXI1 antibody produced in rabbit
SIGMA/AV31403 - affinity isolated antibody
Synonym: Anti-MAD2; Anti-MAX interactor 1; Anti-MGC43220; Anti-MXD2; Anti-MXI
Product Type: Chemical
Immunohistochemistry Anti-MXI1: Cat. No. AV31403: Immunohistochemistry of MXI1 in Human Lung tissue with MXI1 antibody at 4-8 μg/mL.
Immunohistochemistry Immunohistochemistry of MXI1 in Human Lung with MXI1 antibody at 4-8 μg/mL
Immunoblotting Cell Type: Jurkat (Cat. No. AV31403). Lanes 1. Antibody dilution (concentration) 1.0 μg/mL in cell type Jurkat
Western Blotting Western Blot of MXI1 in Mouse Brain with MXI1 antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 26 kDa |
| NCBI accession no. | NP_569157   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, guinea pig, dog, bovine, human, mouse, rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P50539 |
| Application: | Rabbit Anti-MXI1 antibody can be used for western blot applications at 1μg/ml. |
| Biochem/physiol Actions: | Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The MXI1 gene encodes a transcriptional repressor protein thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in MXI1 are frequently found in patients with prostate tumors.Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Max interactor 1 (MXI1) is a bHLH-Zip containing protein that associates with Max to bind Myc-Max recognition sites. MXI1 mutations have been linked to prostate cancer. Rabbit Anti-MXI1 antibody recognizes human, mouse, rat, chicken, bovine, zebrafish, and canine MXI1. |
| Immunogen: | Synthetic peptide directed towards the middle region of human MXI1 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: LNKAKAHIKKLEEAERKSQHQLENL |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |