Anti-SPIB antibody produced in rabbit
SIGMA/AV31422 - affinity isolated antibody
Synonym: Anti-Spi-B transcription factor (Spi-1/PU.1 related)
Product Type: Chemical
Immunoblotting Cell Type: Daudi (Cat. No. AV31422). Lanes 1. Antibody dilution (concentration) 0.25 μg/mL in cell type Daudi
Western Blotting Western Blot of SPIB in Human spleen with SPIB antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 19 kDa |
| NCBI accession no. | NP_003112   |
| shipped in | wet ice |
| species reactivity | bovine, dog, rabbit, guinea pig, human, rat, horse, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: suitable |
| UniProt accession no. | Q01892 |
| Application: | Rabbit Anti-SPIB antibody can be used for western blot applications at 0.25μg/ml. |
| Biochem/physiol Actions: | SPI1 (MIM 165170) and SPIB are members of a subfamily of ETS (see ETS1; MIM 164720) transcription factors. ETS proteins share a conserved ETS domain that mediates specific DNA binding. SPIB and SPI1 bind to a purine-rich sequence, the PU box (5-prime-GAGGAA-3-prime). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | SPIB is a transcriptional stimulator that associates with PU-box and functions as a lymphoid enhancer. SPIB variants have been linked to primary biliary cirrhosis. Rabbit Anti-SPIB antibody recognizes zebrafish, canine, pig, bovine, chicken, human, mouse, and rat SPIB. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human SPIB |
| Other Notes: | Synthetic peptide located within the following region: GQQKGNRKRMTYQKLARALRNYAKT |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |