Anti-RNF12 antibody produced in rabbit
SIGMA/AV32151 - affinity isolated antibody
Synonym: Anti-Ring finger protein 12
Product Type: Chemical
Immunohistochemistry Anti-RNF12: Cat. No. AV32151: Immunohistochemistry of RNF12 in Human Spermatophore tissue with RNF12 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: Jurkat (Cat. No. AV32151). Lanes 1. Antibody dilution (concentration) 2.0 μg/mL in cell type Jurkat
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 69 kDa |
| NCBI accession no. | NP_057204   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rabbit, pig, dog, bovine, human, horse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q9NVW2 |
| Application: | Rabbit Anti-RNF12 antibody can be used for western blot applications at a concentration of 2μg/ml. It can also be used for IHC assays at 4-8μg/ml using paraffin-embedded tissues. |
| Biochem/physiol Actions: | RNF12 is a RING-H2 zinc finger protein. It has been shown to be a ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Alternatively spliced transcript variants encoding the same protein have been reported. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | RNF12 is known to modulate the inactivation of X chromosome in mouse embryonic stem (ES) cells. RNF12 can target Smad7 for degradation, and regulate the cell fate and morphogenesis in zebrafish embryos. Rabbit Anti-RNF12 antibody recognizes human, canine, mouse, chicken, bovine, and rat RNF12. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human RNF12 |
| Other Notes: | Synthetic peptide located within the following region: AQFFLLNEDDDDQPRGLTKEQIDNL |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |