Anti-EYA3 antibody produced in rabbit
SIGMA/AV32331 - affinity isolated antibody
Synonym: Anti-Eyes absent homolog 3 (Drosophila)
Product Type: Chemical
Immunohistochemistry Anti-EYA3: Cat. No. AV32331: Immunohistochemistry of EYA3 in Human Skeletal Muscle tissue with EYA3 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: HepG2 (Cat. No. AV32331). Lanes 1. Antibody dilution (concentration) 0.05-1.0 μg/mL in cell type HepG2
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 63 kDa |
| NCBI accession no. | NP_001981   |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q99504 |
| Application: | Rabbit Anti-EYA3 antibody can be used for IHC (4-8μg/ml) and western blot (0.05-1.0μg/ml) applications. |
| Biochem/physiol Actions: | Eyes absent homolog 3 (EYA3) is a member of the eyes absent (EYA) family of proteins. This protein may act as a transcriptional activator and have a role during development. A similar protein in mice can act as a transcriptional activator. Two transcript variants encoding distinct isoforms have been identified for this gene. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | EYA3 interacts with Six1 and subsequently activates TSHβ expression in response to light changes in the photoperiodic system of mice. EYA3 expression has also been implicated in Ewing sarcoma. Rabbit Anti-EYA3 antibody recognizes human, mouse, rat, and canine EYA3. |
| Immunogen: | Synthetic peptide directed towards the middle region of human EYA3 |
| Other Notes: | Synthetic peptide located within the following region: QSRKNMTSKNRGKRKADATSSQDSE |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |