Anti-SIRT5 antibody produced in rabbit
SIGMA/AV32390 - affinity isolated antibody
Synonym: Anti-Sirtuin (silent mating type information Regulation 2 homolog) 5 (S. cerevisiae)
Product Type: Chemical
Immunohistochemistry Anti-SIRT5: Cat. No. AV32390: Immunohistochemistry of SIRT5 in Human Spermatophore tissue with SIRT5 antibody at 4-8 μg/mL.
Immunohistochemistry Anti-SIRT5: Cat. No. AV32390: Immunohistochemistry of SIRT5 in Human Spermatophore tissue with SIRT5 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: HepG2 (Cat. No. AV32390). Lanes 1. Antibody dilution (concentration) 2.0 μg/mL in cell type HepG2
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 32 kDa |
| NCBI accession no. | NP_036373   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | goat, guinea pig, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q9NXA8 |
| Application: | Rabbit Anti-SIRT5 antibody can be used for western blot (1-2μg/ml) and IHC (4-8μg/ml, using paraffin-embedded tissues) applications. |
| Biochem/physiol Actions: | SIRT5 (Sirtuin 5) is associated with metabolism and aging process. It has been reported in a study that SIRT5 influences urea cycle pathway by NAD-dependent deacetylation and subsequent activation of carbamoyl phosphate synthetase 1 (CPS1), which plays a vital role in the initial step of urea cycle for the detoxification and removal of ammonia. In non-small cell-lung cancer cells. SIRT5 contributions have also been observed for the cancer cell growth and drug resistance. Study shows that SIRT5 may possess oncogenic property. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | SIRT5 (Sirtuin 5) is a mitochondrial matrix protein belonging to the class III of the sirtuin family. It is localized inside the mitochondria and within the cytosol and nucleus. The protein is widely expressed in the brain, heart, liver and kidney. It is composed of very short amino and carboxyl terminal sequences flanking region with the conserved, catalytic sirtuin center domain. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human SIRT5 |
| Other Notes: | Synthetic peptide located within the following region: EVDRELAHCDLCLVVGTSSVVYPAA |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |