Anti-LEF1 (AB1) antibody produced in rabbit
SIGMA/AV32404 - affinity isolated antibody
Synonym: Lef1 Antibody; Lef1 Antibody - Anti-LEF1 (AB1) antibody produced in rabbit; Anti-Lymphoid enhancer-binding factor 1
Product Type: Chemical
Immunofluorescence Tissue: Spermatophore (Cat. No. AV32404). Specification 1. Antibody dilution (concentration) 4-8 μg/mL
Immunoblotting Cell Type: HepG2 (Cat. No. AV32404). Lanes 1. Antibody dilution (concentration) 0.1-2.0 μg/mL in cell type HepG2
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 44 kDa |
| NCBI accession no. | NP_001124185   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, dog, bovine, goat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: suitable |
| UniProt accession no. | Q9UJU2 |
| Application: | Rabbit Anti-LEF1 (AB1) antibody can be used for IHC (4-8μg/ml) and western blot (0.1-2.0μg/ml) applications. |
| Biochem/physiol Actions: | Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | LEF1 is a transcription factor that interacts with β-catenin and modulates cell adhesion. This transcription factor also mediates nuclear responses via the Wnt signaling pathway. Rabbit Anti-LEF1 (AB1) antibody recognizes chicken, human, mouse, rat, canine, rabbit, bovine, zebrafish, and pig LEF1. |
| Immunogen: | Synthetic peptide directed towards the middle region of human LEF1 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: ADIKSSLVNESEIIPASNGHEVARQ |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |