Anti-TAF1 antibody produced in rabbit
SIGMA/AV32473 - affinity isolated antibody
Synonym: Anti-BA2R; Anti-CCG1; Anti-CCGS; Anti-DYT3; Anti-KAT4; Anti-NSCL2; Anti-OF; Anti-TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250 kDa
Product Type: Chemical
Immunohistochemistry Anti-TAF1: Cat. No. AV32473: Immunohistochemistry of TAF1 in Human Kidney tissue with TAF1 antibody at 4-8 μg/mL.
Immunoblotting Cell Type: HeLa (Cat. No. AV32473). Lanes 1. Antibody dilution (concentration) 0.5 μg/mL in cell type HeLa
Western Blotting Western Blot of TAF1 in Mouse Heart with TAF1 antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 215 kDa |
| NCBI accession no. | NP_004597   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | bovine, dog, rat, pig, human, rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ChIP: suitable |
| immunohistochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | P21675-2 |
| Application: | Rabbit Anti-TAF1 antibody has been used for western blotting applications at 0.5μg/ml. |
| Biochem/physiol Actions: | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF1 encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conj |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | TAF1 is a transcription factor that is associated with TATA-binding proteins. It interacts with GCN5 and HD1 and facilitates the histone acetylation needed for light-induced genetic responses. Rabbit Anti-TAF1 antibody recognizes canine, human, mouse, and rat TAF1. |
| Immunogen: | Synthetic peptide directed towards the C terminal region of human TAF1 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: YEVSEEEEDEEEEEQRSGPSVLSQV |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |