Anti-GFI1 (AB2) antibody produced in rabbit
SIGMA/AV32557 - affinity isolated antibody
Synonym: Anti-Growth factor independent 1 transcription repressor; Anti-ZNF163
Product Type: Chemical
Immunoblotting Cell Type: fetal lung (Cat. No. AV32557). Lanes 1. Antibody dilution (concentration) 1 μg/mL in cell type fetal lung
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 45 kDa |
| NCBI accession no. | NP_001120687   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | pig, bovine, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: suitable |
| UniProt accession no. | Q99684 |
| Application: | Rabbit Anti-GFI1 (AB2) antibody can be used for western blot applications at a concentration of 1μg/ml. |
| Biochem/physiol Actions: | GFI1 may be a transcription factor involved in regulating the expression of genes active in the S phase during cell cycle progression in T-cells. GFI1 may be involved in tumor progression. Defects in GFI1 are a cause of autosomal dominant severe congenital neutropenia (SCN) and dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Gfi-1 is a zinc finger repressor that inhibits the proliferation and conserves functional integrity of hematopoietic stem cells. Human GFI1 mutations have been linked to neutropenia. Rabbit Anti-GFI1 (AB1) antibody recognizes human, mouse, rat, and canine GFI1. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human GFI1 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: VPAPSRADSTSNAGGAKAEPRDRLS |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |