Anti-PCK1 (AB2) antibody produced in rabbit
SIGMA/AV32801 - affinity isolated antibody
Synonym: Anti-MGC22652; Anti-PEPCK-C; Anti-PEPCK1; Anti-PEPCKC; Anti-
Product Type: Chemical
Immunofluorescence Tissue: kidney (Cat. No. AV32801). Specification 1. Antibody dilution (concentration) 4-8 μg/mL
Immunoblotting Cell Type: Jurkat (Cat. No. AV32801). Lanes 1. Antibody dilution (concentration) 1.25 μg/mL in cell type Jurkat
Western Blotting Western Blot of PCK1 in Human MCF7 with PCK1 antibody at 1 μg/mL
Western Blotting Western Blot of PCK1 in Human Fetal Heart with PCK1 antibody at 1 μg/mL
Western Blotting Western Blot of PCK1 in Human Fetal Lung with PCK1 antibody at 1 μg/mL
Western Blotting Western Blot of PCK1 in Human Fetal Liver with PCK1 antibody at 1 μg/mL
Western Blotting Western Blot of PCK1 in Human Fetal Muscle with PCK1 antibody at 1 μg/mL
Western Blotting Western Blot of PCK1 in Human Adult Placenta with PCK1 antibody at 1 μg/mL
Western Blotting Western Blot of PCK1 in pig serum with PCK1 antibody at 1 μg/mL
Western Blotting Western Blot of PCK1 in pig serum protein with PCK1 antibody at 1 μg/mL
Western Blotting Western Blot of PCK1 in Human MCF7 Whole Cell with PCK1 antibody at 1 μg/mL
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 69 kDa |
| NCBI accession no. | NP_002582   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, horse, dog, rat, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: suitable |
| UniProt accession no. | P35558 |
| Application: | Rabbit Anti-PCK1 (AB2) antibody can be used for western blot applications at a concentration of 1.25μg/ml. It can also be used for IHC at 4-8μg/ml using paraffin-embedded tissues. |
| Biochem/physiol Actions: | PCK1 is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of PCK1 can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet.This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | PCK1 is a cytosolic enzyme that catalyzes the synthesis of phosphoenolpyruvate from oxaloacetate. Studies in mice have reported that Pck1 may have implications in diabetes and obesity-related disorders. Rabbit Anti-PCK1 (AB1) antibody recognizes bovine, chicken, pig, human, mouse, and rat PCK1. |
| Immunogen: | Synthetic peptide directed towards the middle region of human PCK1 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: NGFFGVAPGTSVKTNPNAIKTIQKN |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |