Anti-SIAH1 (AB1) antibody produced in rabbit
SIGMA/AV34163 - IgG fraction of antiserum
Synonym: Anti-Seven in absentia homolog 1 (Drosophila)
Product Type: Chemical
Immunohistochemistry SIAH1 Antibody: Cat. No. AV34163: Immunohistochemistry analysis of SIAH1 in Human Heart with SIAH1 Antibody Antibody Concentration: 4.0-8.0 μg/mL. Cellular Data: Epithelial cells of renal tubule
Immunoblotting Cell Type: Jurkat (Cat. No. AV34163). Lanes 1. Antibody dilution (concentration) 1.25 μg/mL in cell type Jurkat
Western Blotting Western Blot of SIAH1 in Human HEK293T with SIAH1 antibody at 1 μg/mL
Western Blotting Western Blot of SIAH1 in Mouse Liver with SIAH1 antibody at 1 μg/mL
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 35 kDa |
| NCBI accession no. | NP_001006611   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | guinea pig, bovine, mouse, rabbit, rat, dog, human, horse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | Q8IUQ4-2 |
| Application: | Rabbit Anti-SIAH1 (AB1) antibody can be used for western blot applications at 1.25 μg/ml. |
| Biochem/physiol Actions: | SIAH1 is a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson′s disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | SIAH1 is an E3 ligase that regulates protein ubiquitination and proteasome-mediated degradation. Studies have reported that the N-terminal RING domain regulates proteolysis, whereas the C-terminal sequences modulate target protein binding activities. It has also been implicated in p53 response-mediated degradation of β-catenin. Rabbit Anti-SIAH1 (AB1) antibody recognizes bovine, human, mouse, rat, canine, and zebrafish SIAH1. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human SIAH1 |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: FTCLPAARTRKRKEMSRQTATALPT |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |