Anti-FREQ antibody produced in rabbit
SIGMA/AV34315 - affinity isolated antibody
Synonym: Anti-Frequenin homolog (Drosophila)
Product Type: Chemical
Immunohistochemistry Anti-FREQ: Cat. No. AV34315: Immunohistochemistry of FREQ in Human Brain tissue with FREQ antibody at 4-8 μg/mL.
Immunoblotting Cell Type: HepG2 (Cat. No. AV34315). Lanes 1. Antibody dilution (concentration) 0.5 μg/mL in cell type HepG2
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.5 mg - 1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | 22 kDa |
| NCBI accession no. | NP_055101   |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, human, mouse, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable |
| western blot: suitable | |
| UniProt accession no. | P62166 |
| Application: | Rabbit Anti-FREQ antibody is suitable for western blot (0.5 μg/ml) and IHC (4-8 μg/ml) applications. |
| Biochem/physiol Actions: | FREQ is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. This protein is thought to be associated with secretory granules and may be involved in the regulation of neurosecretion. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | FREQ (NCS1) is a neuronal calcium sensor that modulates G protein-coupled receptor (GPCR) phosphorylation and synaptic functions. Interaction between variation in the DRD2 and FREQ genes can be used to predict the efficacy of nicotine replacement therapy. Rabbit Anti-FREQ antibody recognizes bovine, human, mouse, rat, chicken, zebrafish FREQ. |
| Immunogen: | Synthetic peptide directed towards the N terminal region of human FREQ |
| Physical form: | Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Sequence: | Synthetic peptide located within the following region: MGKSNSKLKPEVVEELTRKTYFTEK |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |